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These effects impair oxygen and nutrient delivery to tissues, triggering hypoxia, metabolic acidosis, and organ failure (Higashi et al., 2014

After 48 h, the measurement was started by switching from DMEM medium to preheated glucose starvation minimal medium (140 mM NaCl, 5 mM KCl, 1 mM of MgCl 2 , 2 mM of CaCl 2 , 20 mM HEPES, pH 7.4 adjusted with NaOH) with 10% FCS

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