Jayaraman, and Larry Kershner Journal of the American Chemical Society 1966 88 (14), 3373-3375 DOI : 10.1021/ja00966a034 Mechanistic study of basic hydrolysis of amides supports a two step addition-elimination mechanism
10.1007/s12192-016-0693-5 89
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To Eliminate non-specific adsorbents caused by hydrophobic adsorption: Wash with 3-4 column bed volumes of 70% ethanol or 2 column bed volumes of 1% Triton X-100, followed immediately by 5 column bed volumes of PBS, pH 7.4.