Seeds were exposed to 0, 50, 100, 150 and 200 mM sodium chloride (NaCl) to assess tolerance thresholds
There is no complete list of banned substances
Unless indicated otherwise, cystine-free medium was prepared by supplementing cystine-, methionine- and glutamine-free DMEM (Gibco, CA, USA) with 100 M methionine, 4 mM glutamine, 1 mM sodium pyruvate, 0.2% bovine serum albumin (BSA), 100 U/ml penicillin and 100 g/ml streptomycin
Intramuscular injections can sometimes be more painful due to going into the muscle belly
The inclusion of Vitamin C and Zinc is crucial for optimizing immune system function, helping the body ward off common infections
The plate was then incubated on a shaker at 900 rpm for 30 min at room temperature, permitting binding of the proteins to the beads