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Preparation of Isolated GCs As previously reported [16], GCs were isolated by enzymatic digestion of retinal tissue with papain and mechanical separation

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A total of 17.6 l of the transfection mix was added to each well already containing 3.6 l siRNA (or 3.6 l water for mock controls), and the mixture was incubated at room temperature for 30 min

*Correspondence: Jonas Michael Bodanowitz, [email protected] Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers