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In the current study, we show that QMP engineering for enhanced pH-dependent FcRn engagement extends the plasma half-life of albumin fusion proteins far beyond that achieved by using WT albumin

In brief, for the HITI donor vector containing the sgAlb-E14 expression cassette and scExe4 donor fragment, the sgAlb-E14 expression cassette and scExe4 (NGF-FCS2-Exe4) sequence flanked by two sgAlb-E14 gRNA target sequences were prepared by PCR using PrimeSTAR GXL polymerase (Takara Bio)

Rotating injection sites may help avoid scar tissue buildup

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