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PMID: 26275694

The role of dimethylarginine dimethylaminohydrolase in idiopathic pulmonary fibrosis

coli over-expression vectors have fusion tags, the translational initiation site, the 5 untranslated region (5 UTR), the antibiotic selection marker, the transcription promoter site, and the origin of replication, just like any other expression vector

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#4: Valproic acid or phenobarbital (whichever you didnt choose as #3)

There is a significant amount of research that has gone into the study of RFA to ensure that it consistently works and remains safe: Long term data RFA resulted in complete eradication of disease in 98% of NDBE patients, with 2.5-years follow-up