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n = 5, both males and females), mice were killed by cervical dislocation, and the glabrous skin from one hind paw with the tibial nerve attached was dissected and placed in a custom-made two-compartment Teflon recording chamber with the corium side down

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Permeabilization was then performed using PBS containing 0.5% Triton X-100 (108,603, Merck, Darmstadt, Germany) for 1 h at room temperature, followed by washing twice for 5 min in PBS/BSA

Brain Function : L-carnitine is involved in brain metabolism and may have neuroprotective effects