Luminescence was recorded for 45 min at 1 min intervals using a Fluoroskan Ascent FL (Thermo Scientific, Rockford, IL, USA) fluorimeter (excitation: 538 nm, emission: 604 nm), and the slopes were used for calculations
We often look at external factors like room temperature or blue light, but the real key to better rest might be happening at the cellular level
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1.2 BCA protein concentration measurement (1) Prepare the BSA standard and prepare BCA working solution A: B = 50:1
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This study was performed to evaluate this hypothesis