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From Day 17 onward, liver organoids were matured in Leibovitz L15 medium supplemented with Dihexa (100 nM), Dexamethasone (100 nM), Hydrocortisone (10 M), Sodium L-ascorbate (50 g/mL), Insulin-transferrin-selenium (0.58%), FBS (8.3%), Glutamax, and BME
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These could provide insights on why some variants may cause a higher degree of phenotypic severity compared to others
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The STAT3 factors induce the expression of SOCS proteins which consequently inhibit the function of JAK/STAT3 signaling, i.e., SOCS proteins exert a negative feedback on the JAK/STAT3 signaling induced by both insulin/IGF-1 and certain cytokine pathways
