doi: 10.1016/S0140-6736(22)02076-1 73 Rivera-MartnezWMeja-CardonaASalazar-SolarteAAristizabal-ColoradoDGarces-VillabonLPinillos-SeniorOet al
Journal International De Vitaminologie Et De Nutrition
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Generation and quantification of persulfides on Cofilin1 Purified full-length human recombinant Cofilin 1 protein expressed in Escherichia coli (Abcam #62958) (11 g/sample) was incubated with GSH (1 mM), MitoPerSulf (100 M) or both in 50 mM HEPES buffer (pH 7.4) for 7 min at RT, followed by alkylation with IAM-TPP (20 mM) at RT for 30 min in the dark
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