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Beads were then washed three times with binding buffer, and bound complexes were eluted in 20 mM Tris-HCl pH 8.0, 150 mM NaCl, 250 mM imidazole elution buffer

For a comprehensive safety overview, see our glutathione side effects guide

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If you look at the clinical trial data, typically its 30% to 40% of patients experience those side effects

K., Palit, A., Parkman, H

however, emerging evidence suggests it may also mediate Mn uptake