The first injection in port A contained the substrate for each individual respiratory chain complex (I: 5 mM pyruvate + 2.5 mM malate, II: 10 mM succinate + 1 M rotenone, III: 0.5 mM duroquinol, IV: 0.5 mM TMPD + 2 mM ascorbic acid) along with 1 mM ADP and 10 nM recombinant perfringolysin O (Agilent Technologies)
For most people, daily oral methylcobalamin is more practical than chasing B12 energy shots, which are really designed for confirmed deficiency or malabsorption
Ask Before Injecting Contact your provider or dispensing pharmacy before the injection when the dose, concentration, syringe markings, preparation method, or injection instructions are unclear
10.1007/s00262-008-0524-3 78 GrnewaldS.PolitzO.BenderS.HroultM.LustigK.ThussU.et al (2019)
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