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The reduction of roGFP2(S 2 ) is therefore a good example for an enzyme using an alternative reaction pathway to overcome a kinetic challenge, and we hypothesize that other non-glutathione disulfide substrates with reactive proximal cysteinyl residues and rather negative redox potentials also require a ternary complex for their reduction

H-L) Time course and respective F/F 0 values for POMC-GFP+ neurons (highlighted in green) and GFP-neurons stimulated initially with semaglutide and then with the VGCC blockers -Agatoxin (H), TTA-P2 (I), benidipine (J), nifedipine (K) and combined -Agatoxin + TTA-P2 + benidipine (L)

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