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Real-time qPCR was performed using an Applied Biosystems QuantStudio 12 K Flex real time PCR system (Thermo Fisher Scientific, San Jose, CA, USA) with THUNDERBIRD SYBR qPCR Mix (Toyobo), 6 pmol of primers, and aliquots of cDNA

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Components: MOTS-c: 5mg subcutaneous every 3 days CJC-1295 (no DAC): 150mcg subcutaneous before bed Ipamorelin: 150mcg subcutaneous before bed Schedule: MOTS-c every third day

Randomized trials of semaglutide for chronic weight management have demonstrated substantial mean weight reductions when combined with structured lifestyle intervention