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For analyses of GLP-1, glucagon, and Ex9-39, blood was sampled into chilled tubes containing EDTA and a specific DPP-4 inhibitor (3 mmol/l valine-pyrrolidide, 10 l/ml blood, a gift from Novo Nordisk) and kept on ice until centrifugation

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Using sterile water (which has no preservative) is an option only if you plan to use the entire vial in a single session, which is rarely practical

This study is part of the PE3 RETURN project Task 5.4.4 - Circular Metabolism in Urban and Metropolitan Settlements.Speaker: Dr Bruna Vendemmia (DiARC Universit degli Studi di Napoli Federico II) 164 Ecopolis

Chromatogram: The visual output of an HPLC test showing the purity peaks