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After treatment, to assess mitochondrial membrane potential ( m ), A375 cells were incubated with 4 M JC-1 (ThermoFisher Scientific) in phenol red free DMEM for 20 min in a 5% CO 2 incubator at 37 C, then washed twice with DMEM without phenol red

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10.1159/000207822 Acta Haematol