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Cell culture and treatment FL83B mouse hepatocytes (ATCC , Manassas, VA) were cultured and maintained in F-12K complete medium

Finally, the MethylFlash Global DNA Methylation (5mC) ELISA Easy Kit (colorimetric) was used according to the manufacturers instructions, to assess global levels of 5-methylcytosine (5mC)

Neuropharmacology 47(1):4664

J Ethnopharmacol 162:6978

human anecdotal dosing is variable, often 250-500 mcg per day (subQ) for BPC-157, and TB-500 perhaps a few milligrams per week split across injections

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