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Results TGP was brighter than the conventional GFPs Two GFP variants, commonly used as fusion tags in Escherichia coli 50,60 (ecGFP, NCBI AGT98535.1 ) and Saccharomyces cerevisiae 27 , insect cells 61,62 , and mammal cells 63 (scGFP, NCBI ABI82039.1 ), were used for comparison