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Collected the supernatant and precipitated it with isopropanol

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Methods NB organoid culture LU-NB organoids were previously established from LU-NB PDXs 1, 2, 3 and 3-Relapsed (Table S1) 12,24,25 and cultured as free-floating 3D organoids under serum-free conditions in low glucose Dulbeccos modified Eagles medium (ThermoFisher, 21885) and GlutaMAX F-12 Nut Mix (ThermoFisher, 31765027) in a 3:1 ratio, supplemented with 1% penicillin/streptomycin (Corning, 30-002-CI), 2% B27 without vitamin A (ThermoFisher, 12587001), fibroblast growth factor (40 ng/ml) (Peprotech, AF-100-15), and epidermal growth factor (20 ng/ml) (Peprotech, AS-100-18B)

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