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Nat Genet 19:379383 Mariadason JM, Bordonaro M, Aslam F, Shi L, Kuraguchi M, Velcich A et al (2001) Down-regulation of -catenin TCF signaling is linked to colonic epithelial cell differentiation

500 ng of DNA was treated with bisulfite using the EZ DNA Methylation kit from Zymo Research, following the manufacturers instructions