Bibcode:1983Natur.306..284T
This technological breakthrough has rendered the molecular defect characteristics of tumor cells the groundwork for clinical diagnosis and treatment, significantly enhancing the scientific and precise nature of treatment plans
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doi: 10.1016/s0034-5288(96)90074-0
Untargeted Metabolomics analysis Untargeted metabolomics profiling was performed by Metabo-Profile (Shanghai, China) following these steps: centrifugation, extraction, evaporation, lyophilization, derivatization, and injection
The resulting pellet was re-suspended in minimal 50 mM HEPES pH 8.0, 200 mM NaCl, applied to a 1545% w/v sucrose gradient and subjected to ultracentrifugation at 4 C for 3 h, 105,000 g