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Glucagon and total GLP-1 concentrations were measured using V-PLEX assays (Glucagon: K150U5D, GLP-1 (total): K1503PD)

For long-term treatment, incorporating B vitamins into the feed using swine vitamin premix or top-dressing with supplements like Show Bloom is an option, though direct administration remains the most reliable method

Cells were then lysed with respective lysis buffers provided in the kits and the abundance of metabolites was determined according to the manufacturers protocols

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