Ethics declarations Ethics approval and consent to participate All procedures used for this study were approved by University of Nebraska Medical Center Institutional Animal Care and Use Committee (IACUC, approval reference number: 14-036-07-FC) and conducted according to the National Institutes of Health guiding principles for the research involving animals
Lipid aldehyde staining In total, 30,000 (Pfa1 xCT OE) or 250,000 cells (HeLa) per well were seeded into six-well plates in DMEM with 10% FCS or in FluoroBrite with 2% FCS
Morphological, molecular analysis of tissue sections and of retina total protein extracts Sample preparation Eyes were removed and the corneas were eliminated at limbus level
Study 2 ( N = 706) examined stigma directed at people who regain weight after discontinuing GLP-1s, and the target was described as either: having regained weight after discontinuing a GLP-1, having regained weight after discontinuing a diet and exercise plan, not having lost weight, or having maintained weight loss
However, its impact on appetite is where semaglutide truly shines
Feb 2017;15(2):257-265.e253