Oxidised glutathione (GSSG) and the GSH precursor, gGlu-Cys, were both significantly elevated in treated cells, while reduced GSH was decreased (Fig 7C)
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In both cases, changes to physical properties of the environment can lead to changes in dye fluorescence properties, e.g., changes to excitation and/or emission maxima, extinction coefficient, Stokes shift, and/or fluorescence lifetime
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Apoptosis, on the other hand, is distinguished by the cleavage of certain substrates and the activation of caspases