The plasmid for the non-tagged SUN2 expression was cloned by cutting the SUN2-CFP plasmid 65 with EcoRI and BamHI, blunting with Phusion DNA Polymerase (New England Biolabs, M0530), and re-ligating the plasmid thus introducing a stop codon in frame with the SUN2 sequence
Pedrazzini T, Pralong F, Grouzmann E (2003) Neuropeptide Y: the universal soldier
Also known as: ascorbic acid A powerful antioxidant, vitamin C may protect sperm from damage
this peptide is not currently prescribable through legitimate compounding pharmacies
Potter WZ, Davis DC, Mitchell JR, Jollow DJ, Gillette JR, Brodie BB: Acetaminophen-induced hepatic necrosis
Blueprint is rooted in the desire to place oneself on a trajectory of exponential growth