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It induces purified mitochondria to release the apoptogenic proteins cytochrome c and caspase-9

PKC also triggers the closure of ATP-dependent K + channels and the activation of TRPM4/5 channels

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These four compounds additive and partially synergistic effects with BRAFi or MEKi

The supernatant was aspirated and the cell pellet was suspended in supplemented DMEM media for further experiments