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Engineering a High-Affinity Scaffold for Non-Chromatographic Protein Purification via Intein-Mediated Cleavage

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When NRX is oxidized by NADPH oxidase 1 (NOX1)-derived H2O2 or lipid peroxide (ROOH), DVL is released and inhibits glycogen synthesis kinase 3 (GSK3) in the -catenin degradation complex, thereby stabilizing the -catenin in the cytoplasm and partially entering the nucleus to activate the target gene GPX2 by correlation with the TCF/LEF transcription factor family (Kipp et al., 2012) (Figure 2)

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