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When the BioID ligase is fused to the target protein and expressed in cells, it converts exogenously added free biotin into a highly reactive but unstable biotin-5-AMP, which is released from the enzymes active site and reacts with nearby protein amines

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doi: 10.1111/j.1749-6632.2002.tb02098.x 59 DhanasekaranAKotamrajuSKalivendiSVMatsunagaTShangTKeszlerAet al

Angew Chem Int Ed 47(33):61726176 Zhang H, Liu R, Liu J, Li L, Wang P, Yao SQ, Xu Z, Sun H (2016) A minimalist fluorescent probe for differentiating Cys, Hcy and GSH in live cells