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You've experienced severe reactions like significant allergic response or dramatic worsening
The probe performs similarly to non-modified SNAP-Lumi4-Tb for GLP-1R labelling, measurements of fluorescent GLP-1R agonist binding and for internalisation by DERET (Supplementary Figure 2B, 2C, 2D)
To isolate chondrocytes, fresh articular cartilage tissues were rinsed with basal medium (BM), consisted of high glucose Dulbeccos modified Eagles medium (DMEM, Gibco/Thermo Fisher Scientific, Waltham, MA, United States), with 2 Antibiotics-Antimycotics (anti-anti, Life Technologies, Carlsbad, CA, United States) and then cut into 1 mm 3 pieces
This results in them eating less leading to quick and often quite dramatic weight loss
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