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The release of free cobalamin from the lysosome requires the action of the lysosomal membrane protein identified as LMBRD1 (LMBR1 domain containing 1, where LMBR1 refers to the protein identified as limb region 1 protein homolog)

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The bone marrow was flushed out by LG-DMEM with a 20-gauge needle attached to a 2 mL syringe

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