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It has previously been suggested that the GT II assay is able to reliably detect mixed infections if the minor genotype/subtype represents at least 2030% of the circulating variants (similarly to Sanger sequencing) 13,35

Nat Commun 16(1):867 Liang D, Feng Y, Zandkarimi F et al (2023) Ferroptosis surveillance independent of GPX4 and differentially regulated by sex hormones

To soothe immediate irritation, you can apply over-the-counter hydrocortisone cream to the affected area.

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Its important to follow a proper dosage schedule and receive guidance from a medical professional