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8E), the VPA-ASD group was characterized by a marked increase in the relative abundance of Pseudomonadota (Proteobacteria) and Deferribacterota, alongside a severe depletion of Bacteroidota and Verrucomicrobiota compared to Controls

Quantitative real-time PCR was performed using SYBR Green (SYBR Select Master Mix, Applied Biosystems) on a ViiA7 system (Applied Biosystems), and results were analyzed using the 2ddCt methods

J Gene Med (2006) 8(6):76472

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