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2.3 Experimental protocol Following 1 week of acclimation, six rats were allocated randomly to one of four groups: Rats in Group 1, the control group, were given saline intraperitoneally (IP) twice weekly for 6 weeks
For calcium-dependent aggregation assays, Syn WT (50 M) was pre-incubated with 3.0 mM CaCl for 30 min prior to initiating aggregation, as previously described 102
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