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Preoperative fasting guidelines, such as those set by the American Society of Anesthesiologists (ASA), are designed to minimize the volume of residual gastric contents (Joshi et al
A Dionex UltiMate 3000 506 LC System (Thermo Scientific) and a Q Exactive Orbitrap mass spectrometer (Thermo Scientific) operated in negative mode was used for targeted measurement
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Once reconstituted, the solution should be aliquoted and stored at 20C to preserve stability and avoid repeated freeze-thaw cycles, which can degrade peptide integrity
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