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Observe for side reactions when conducting research applications, and record dosages and observations

Location: You'll be injecting into the fatty tissue overlying the deltoid muscle

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After incubation, the beads were washed with 1 ml of ice-cold phosphate-buffered saline (PBS), pH 7.4, three times, and the beads were eluted in sample loading buffer by boiling for 10 min at 100 C