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To test this, we treated Hs 578T cells with amino-oxyacetic acid (AOAA) and I3MT-3, pharmacological inhibitors of endogenous H 2 S-producing enzymes, and observed no decrease in NRF2 expression (Fig

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The tissue was then blotted dry and weighed, followed by homogenization in 1.5 ml cold buffer (50 mM potassium phosphate and 1 mM EDTA pH 7) and centrifugation at 10,000 xg for 15 min at 4C was done