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When increasing concentrations of KA were added in the presence of L-dopa as the substrate, a similar mode of mixed inhibition was observed, with K I of 3.5 M and K IS of 150 M, similar to previous studies that reported K I values of 3.4, 5 and 4.7 M for mushroom tyrosinase 18,19,33

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The simulation applied the Ordinary Differential Equations (ODEs) to create an exploratory model by speculating the system progression and the experimental result [4] (Figure 5)